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Anti-6×His tag antibody [HS1] - Pro Marketing (PMA104)

貨號
PMA104
規格
50μl/100μl/200μl
價格
¥800.00/1200.00/2100.00
 
 
說明書下載

産品優勢

● Recognizes all proteins which contain either an N or C-terminal HHHHHHGS epitope tag. It has been shown to recognise HHHHHH alone.

産品描述

Overview
Product name
Anti-6×His tag antibody [HS1] - Pro Marketing
Description
Mouse monoclonal [HS1] to 6×His tag
Specificity
Recognizes His-tagged recombinant proteins or His-tagged proteins overexpressed in cells.
Tested applications
WB, ICC/IF, IP
Immunogen
6× His synthetic peptide
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer
pH: 7.2, Preservative: 0.1% Sodium azide, Constituent: PBS.
Concentration
100 µg at 1 mg/ml
Purity
IgG fraction
Clonality
Monoclonal
Clone number
HS1
Isotype
IgG2a
Applications
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
1/500 - 1/5000
ICC/IF
1/50 - 1/500
IP
1/50 - 1/200
Target
Relevance
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Cellular localization
Depends upon the localization of the parent protein tagged with hexahistidine.
Alternative names
6 His epitope tag antibody, Hexa His tag antibody, HHHHHH epitope tag antibody, HHHHHH tag antibody, His tag antibody, Polyhistidine Tag antibody, etc.
Anti-6×His tag antibody [HIS-1] - Pro Marketing images
 
All lanes : Recombinant 6×His tag protein produced in E. coli with C-terminal poly Histidine was separated on SDS-PAGE and probed with Anti-6×His tag antibody [HS1] - Pro Marketing (PMA104).
 
Lane 1 : Antibody dilution 1:1000
Lane 2 : Antibody dilution 1:2000
Lane 3 : Antibody dilution 1:10000
Lane 4 : Negative control: without first antibody
 
Anti-6×His tag antibody [HS1] - Pro Marketing (PMA104)

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