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Anti beta Actin antibody [mPro7] - Loading Control (PMC201)

貨號
PMC201
規格
50μl/100μl/200μl
價格
¥800.00/1200.00/2100.00
 
 
說明書下載

産品優勢

● 高質量的beta Actin小鼠單抗

● WB稀釋比高達1/5000 - 1/50000

● 可用于WB, IHC, ICC/IF, FC, IP, ELISA等實驗

産品描述

 

Overview                                                      
Product name
Anti beta Actin antibody [mPro7] - Loading Control                                        
Description
Mouse monoclonal [mPro7] to beta Actin
Specificity
Does not cross-react with adult cardiac, smooth, or skeletal muscle actin.
Tested applications
WB, IHC, ICC/IF, FC, IP, ELISA
Species reactivity
Reacts with: Human, Mouse, Rat, Hamster, Zebrafish. Other species are not tested, Please decide the specificity by homology.
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human beta Actin.
Positive control
This antibody gave a positive signal in the following whole cell lysates: A431; HEK293; NIH3T3.
General notes
The concentration of this antibody is batch dependent.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer
pH: 7.40, Preservative: 0.02% Sodium azide, Constituent: PBS
Concentration
100 µg at 1 mg/ml
Purity
IgG fraction
Clonality
Monoclonal
Clone number
mPro7
Isotype
IgG2b
Applications
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
1/5000 - 1/50000.
IHC
1/20 - 1/200.
IF
1/20 - 1/200.
IP
1/5000 - 1/50000.
FC
Use at an assay dependent concentration.
Target
Relevance
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Alternative names
ACTB antibody, ACTB_HUMAN antibody, Actin antibody, Actin cytoplasmic 1 antibody, b actin antibody, b-actin antibody, Beta cytoskeletal actin antibody, Beta-actin antibody, etc.
Anti beta Actin antibody [mPro7] - Loading Control images
 
              
 
All lanes : Anti beta Actin antibody [mPro7] - Loading Control (PMC201) at 1/10000 dilution
 
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate.
Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate.
 
Lysates/proteins at 10 µg per lane.
Western blot - Anti beta Actin antibody [mPro7] - Loading Control (PMC201)

 

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